Overview

  • Product nameAnti-PPAR delta antibody
    See all PPAR delta primary antibodies
  • Description
    Goat polyclonal to PPAR delta
  • Specificityab21209 recognises isoform 1 of PPAR delta, but not isoform 2, a shorter protein with a different C-terminus.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide:

    CHPLLQEIYKDMY

    , corresponding to C terminal amino acids 429-441 of Human PPAR delta .

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab21209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

Target

  • FunctionLigand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
  • Tissue specificityUbiquitous with maximal levels in placenta and skeletal muscle.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FAAR antibody
    • MGC3931 antibody
    • NR1C2 antibody
    • NUC1 antibody
    • NUCI antibody
    • NUCII antibody
    • Nuclear hormone receptor 1 antibody
    • Nuclear receptor subfamily 1 group C member 2 antibody
    • Peroxisome proliferative activated receptor delta antibody
    • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
    • Peroxisome proliferator-activated receptor beta antibody
    • Peroxisome proliferator-activated receptor delta antibody
    • PPAR beta antibody
    • PPAR-beta antibody
    • PPAR-delta antibody
    • PPAR-ß antibody
    • PPARB antibody
    • ppard antibody
    • PPARD_HUMAN antibody
    see all

Anti-PPAR delta antibody images

  • Anti-PPAR delta antibody (ab21209) + Jurkat lysate (35µg protein in RIPA buffer).

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References for Anti-PPAR delta antibody (ab21209)

This product has been referenced in:
  • Borland MG  et al. Modulation of aryl hydrocarbon receptor (AHR)-dependent signaling by peroxisome proliferator-activated receptor ß/d (PPARß/d) in keratinocytes. Carcinogenesis N/A:N/A (2014). Read more (PubMed: 24639079) »
  • Borland MG  et al. Stable over-expression of PPARß/d and PPAR? to examine receptor signaling in human HaCaT keratinocytes. Cell Signal 23:2039-50 (2011). WB ; Human . Read more (PubMed: 21843636) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (thymus)
Loading amount 20 µg
Specification thymus
Gel Running Conditions Reduced Denaturing (12 %)
Blocking step BSA as blocking agent for 36 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
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Verified customer

Submitted Mar 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"