Overview

  • Product name
  • Description
    Rabbit polyclonal to PPAR delta
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, Pig
    Predicted to work with: Rabbit, Chicken
  • Immunogen

    Synthetic peptide:

    SSSYTDLSRSSSPPSL

    , corresponding to amino acids 39 - 54 of Human PPAR delta .

  • Positive control
    • WB: Human cerebral cortex lysate, murine liver lysate ICC/IF: HT29 cells Rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab23673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 4 µg/ml.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa.Can be blocked with Human PPAR delta peptide (ab104373). Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
  • Tissue specificity
    Ubiquitous with maximal levels in placenta and skeletal muscle.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FAAR antibody
    • MGC3931 antibody
    • NR1C2 antibody
    • NUC1 antibody
    • NUCI antibody
    • NUCII antibody
    • Nuclear hormone receptor 1 antibody
    • Nuclear receptor subfamily 1 group C member 2 antibody
    • Peroxisome proliferative activated receptor delta antibody
    • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
    • Peroxisome proliferator-activated receptor beta antibody
    • Peroxisome proliferator-activated receptor delta antibody
    • PPAR beta antibody
    • PPAR-beta antibody
    • PPAR-delta antibody
    • PPAR-ß antibody
    • PPARB antibody
    • ppard antibody
    • PPARD_HUMAN antibody
    see all

Images

  • Anti-PPAR delta antibody (ab23673) at 1/1000 dilution + Rat brain tissue lysate at 100 µg

    Secondary
    HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunofluorescent staining of HT-29 cells (ab3952) (nuclear staining) using ab23673.

  • Immunohistochemical analysis of paraffin-embedded pig kidney tissue labeling PPAR delta with ab23673 at 1/100 dilution,followed by Goat Anti-Rabbit IgG

    See Abreview

  • ab23673 staining PPAR delta in Rat brain tissue (ab29475) sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with acetone, permeablized with methanol and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100 in PBS) at 4°C for 18 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit (ab150077) polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab23673 staining mouse brain tissue sections by IHC-Fr.  Sections were PFA fixed and permeabilized in Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C.  The primary antibody was diluted 1/200 and incubated with the sample for 48 hours at 4°C.  A FITC conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-PPAR delta antibody (ab23673)

    Lane 1 : Human cerebral cortex (30ug)
    Lane 2 : Murine liver (30ug)


    Predicted band size : 50 kDa
    ab23673 recognises PPAR delta at 50kDa from human samples. An additional smaller size of PPAR delta (~40kDa) is also detected in certain murine tissues.

References

This product has been referenced in:

See all 15 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Chicken Tissue lysate - whole (Chicken Embryo)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Chicken Embryo
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (Astrocyte Culture)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
1.5 µg
Specification
Astrocyte Culture
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 30 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain cortex)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
20 µg
Specification
brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 29 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate Buffer pH 6.0
Sample
Pig Tissue sections (kidney)
Specification
kidney
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Oscar Mccook

Verified customer

Submitted Sep 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (10% Gel)
Sample
Rat Tissue lysate - whole (brain tissue)
Specification
brain tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Sep 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Permeabilization
Yes - Methanol
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Rat Cell (Astrocytes)
Specification
Astrocytes
Permeabilization
Yes - methanol
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 16 2013

Vielen Dank für Ihre Antwort.

Es tut mir leid, keine eindeutige Lösung gefunden zu haben. In der Tat kann die vorhergesagte Grösse von der beobachteten Grösse ziemlich verschieden sein - es kann bis zu 10% unterschiedlich ...

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Vielen Dank für Ihre ausführliche Illustration. Es tut mir leid, dass Sie Probleme haben mit diesem Antikörper. Falls wir dies Problem nicht lösen können, werden wir Ihnen diesen Antikörper ersetzen. Da uns die Qualität unserer Produkte sehr wichtig is...

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Vielen Dank für Ihren Anruf. Es tut mir leid zu hören, dass Sie Probleme mit dem ab23673 haben. Wie am Telefon diskutiert, würde ich gerne dieses Problem genauer untersuchen. Bitte senden Sie mir hierzu die Bilder und das benutzte Protokoll. Vielen Dan...

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