Anti-PPAR delta antibody (ab8937)
Key features and details
- Rabbit polyclonal to PPAR delta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PPAR delta antibody
See all PPAR delta primary antibodies -
Description
Rabbit polyclonal to PPAR delta -
Host species
Rabbit -
Specificity
This affinity purified antibody is directed against mouse PPAR delta protein. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide:
MEQPQEETPEAREE
, corresponding to N terminal amino acids 1-14 of Mouse PPAR delta . -
Positive control
- 3T3 cell lysate HepG2
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB | (3) |
1/1000 - 1/4000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
1/1000 - 1/4000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
Tissue specificity
Ubiquitous with maximal levels in placenta and skeletal muscle. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5467 Human
- Entrez Gene: 19015 Mouse
- Entrez Gene: 25682 Rat
- Omim: 600409 Human
- SwissProt: Q03181 Human
- SwissProt: P35396 Mouse
- Unigene: 696032 Human
- Unigene: 328914 Mouse
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Alternative names
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
Images
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ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Rabbit polyclonal to PPAR delta (ab8937).
Each lane contains 20 ug 3T3 cell lysateLane 1: ab8937 1/500
Lane 2: ab8937 1/1000Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000
Rabbit polyclonal to PPAR delta (ab8937). Each lane contains 20 ug 3T3 cell lysate (ab7179) Lane 1: ab8937 1/500 Lane 2: ab8937 1/1000 Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab8937 has been referenced in 16 publications.
- Liu Y et al. Rapid acceleration of KRAS-mutant pancreatic carcinogenesis via remodeling of tumor immune microenvironment by PPARδ. Nat Commun 13:2665 (2022). PubMed: 35562376
- Ho SY et al. Investigating the Role of PPARß/d in Retinal Vascular Remodeling Using Pparß/d-Deficient Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32575793
- Luo Y et al. The function of peroxisome proliferator-activated receptors PPAR-? and PPAR-d in Mycobacterium leprae-induced foam cell formation in host macrophages. PLoS Negl Trop Dis 14:e0008850 (2020). PubMed: 33075048
- Mandai S et al. WNK1 regulates skeletal muscle cell hypertrophy by modulating the nuclear localization and transcriptional activity of FOXO4. Sci Rep 8:9101 (2018). PubMed: 29904119
- Hu X et al. MiR-27b Impairs Adipocyte Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting LPL. Cell Physiol Biochem 47:545-555 (2018). PubMed: 29794473