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Our Abpromise guarantee covers the use of ab8937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/1000 - 1/4000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).|
ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Rabbit polyclonal to PPAR delta (ab8937).
Each lane contains 20 ug 3T3 cell lysate
Lane 1: ab8937 1/500
Lane 2: ab8937 1/1000
Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000