Baculovirus-expressed recombinant fragment, corresponding to amino acids 3-105 of Human PPAR gamma 1+2.
Our Abpromise guarantee covers the use of ab41928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform antigen retrieval by autoclave to preserve reproducibility.
Sections should be fixed with formalin for no longer than 48 hours.
|EMSA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. A nuclear extraction is recommended.|
|ELISA||Use a concentration of 0.012 µg/ml.|
|IP||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 16197558|
Lane 1: FLAG-M2
Lane 2: ab41928
Lane 3: ab41928
Lane 4: Control IgG
Immunoprecipitation analysis using Fluorescence labeled PPAR gamma generated in vitro translation. Anti-PPAR gamma antibody (ab41928) is diluted with translation production and mixed with protein G Sepharose beads. It is then rotated at 4oC for 1 hour and precipitates are eluted with addition of SDS loading buffer. Samples are incubated for 10minutes at 70°C and analyzed with Fluorescence imager.