Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade (ab41928)

Overview

  • Product nameAnti-PPAR gamma 1+2 antibody [A3409A] - ChIP GradeSee all PPAR gamma 1+2 primary antibodies ...
  • Description
    Mouse monoclonal [A3409A] to PPAR gamma 1+2 - ChIP Grade
  • SpecificityThis antibody specifically recognizes PPAR gamma 1 and 2. The specificity for each subtype regarding to alpha, gamma -1, -2 , delta was confirmed by each cDNA transfected to CHO cell and the lysate reacted with corresponding antibody in a panel; the antibody was found not to react with alpha or delta forms.
  • Tested applicationsIHC-P, WB, ELISA, IP, ChIP, Gel supershift assaysmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Baculovirus-expressed recombinant fragment, corresponding to amino acids 3-105 of Human PPAR gamma 1+2.

  • Positive control
    • IHC: Rat adipose cells and rat placenta. WB: Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation

Properties

Applications

Our Abpromise guarantee covers the use of ab41928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform antigen retrieval by autoclave to preserve reproducibility.
Sections should be fixed with formalin for no longer than 48 hours.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. A nuclear extraction is recommended.
ELISA Use a concentration of 0.012 µg/ml.
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution. PubMed: 16197558
Gel supershift assays Use at an assay dependent dilution.

Target

  • FunctionReceptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • Tissue specificityHighest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • Involvement in diseaseNote=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HUMPPARG antibody
    • NR1C3 antibody
    • Nuclear receptor subfamily 1 group C member 3 antibody
    • Peroxisome proliferator-activated receptor gamma antibody
    • PPAR gamma antibody
    • PPAR-gamma antibody
    • PPARG antibody
    • PPARG_HUMAN antibody
    • PPARG1 antibody
    • PPARG2 antibody
    see all

Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade images

  • ab41928 staining PPAR gamma 1+2 in rat adiposoe cells (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
  • ab41928 staining PPAR gamma 1+2 in rat plancenta(10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
  • All lanes : Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade (ab41928) at 1 µg/ml

    Lane 1 : Nuclear lysate of non induced 3T3l-1 cells
    Lane 2 : Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 58 kDa

References for Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade (ab41928)

This product has been referenced in:
  • Pepping JK  et al. NOX2 deficiency attenuates markers of adiposopathy and brain injury induced by high-fat diet. Am J Physiol Endocrinol Metab 304:E392-404 (2013). Mouse . Read more (PubMed: 23233541) »
  • Prokesch A  et al. Arxes: retrotransposed genes required for adipogenesis. Nucleic Acids Res 39:3224-3239 (2011). IP ; Mouse . Read more (PubMed: 21177646) »

See all 3 Publications for this product

Product Wall

Proteins that are found exclusively or predominantly in a sub-cellular location can be enriched in a lysate of the sub-cellular fraction compared to whole cell or tissue lysates. This can be useful when trying to obtain a signal for a weakly-expressed ...

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I am very pleased to hear you would like to accept our offer and test ab41928 in Flow Cytometry. This code will give you xxxxxxxxx off your next order before the expi...

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Thank you for contacting us.
Regarding PPAR-delta ab:
I think ab58137 (www.abcam.com/ab58137) as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. Howeve...

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