Anti-PPAR gamma antibody - ChIP Grade (ab45036)

Overview

  • Product nameAnti-PPAR gamma antibody - ChIP Grade
    See all PPAR gamma primary antibodies
  • Description
    Rabbit polyclonal to PPAR gamma - ChIP Grade
  • SpecificityAb45036 detects peroxisome proliferator activated receptor (PPAR) gamma 2. This antibody does not detect PPAR alpha or PPAR delta. This sequence is from P37231-1 (Isoform 2), the sequence is not present in P37231-2 (Isoform 1) or P37231-3 (Isoform 3).
  • Tested applicationsSuitable for: ChIP, IP, IHC-P, ICC/IF, WB, Functional Studies, EMSA, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Non Human Primates
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human PPAR gamma 2 aa 1-16.
    Sequence:

    MGETLGDSPIDPESDSC


    (Peptide available as ab41762)

  • Positive control
    • NIH-3T3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab45036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 21247904
IP Use at an assay dependent concentration. PubMed: 21247904
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.
WB 1/500. Predicted molecular weight: 56 kDa.Can be blocked with Human PPAR gamma peptide (ab41762).

By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate.

Functional Studies Use at an assay dependent concentration. Ab45036 inhibits PPAR gamma 2 DNA binding.
EMSA Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • FunctionReceptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • Tissue specificityHighest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • Involvement in diseaseNote=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIMT1 antibody
    • GLM1 antibody
    • NR1C3 antibody
    • Nuclear receptor subfamily 1 group C member 3 antibody
    • OTTHUMP00000185032 antibody
    • OTTHUMP00000185036 antibody
    • Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody
    • Peroxisome proliferator activated receptor gamma 1 antibody
    • Peroxisome Proliferator Activated Receptor gamma antibody
    • Peroxisome proliferator-activated receptor gamma antibody
    • PPAR gamma antibody
    • PPAR-gamma antibody
    • PPARG antibody
    • PPARG_HUMAN antibody
    • PPARG1 antibody
    • PPARG2 antibody
    • PPARgamma antibody
    see all

Anti-PPAR gamma antibody - ChIP Grade images

  • ab45036 labelling PPAR gamma 2 (green) in the nucleus and cytoplasm of HeLa cells (right) compared with a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat-anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • ab45036 labelling PPAR gamma 2 (green) in the nucleus and cytoplasm of NIH-3T3 cells (right) compared with a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat-anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • ab45036 labelling PPAR gamma 2 (green) in the nucleus and cytoplasm of 3T3-L1 cells (right) compared with a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat-anti-rabbit IgG was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • All lanes : Anti-PPAR gamma antibody - ChIP Grade (ab45036) at 1/1000 dilution

    Lane 1 : NIH-3T3 cell lysate
    Lane 2 : 3T3-L1 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Lane 1 :
    Lane 2 : Anti-PPAR gamma antibody - ChIP Grade (ab45036)

    Lane 1 : Protein Marker
    Lane 2 : Mouse liver tissue lysate at 1 µg

    Secondary
    Lane 2 : IRDye® 680RD Donkey anti-Rabbit


    Performed under reducing conditions.

    Predicted band size : 56 kDa


    Exposure time : 2 seconds

    This image is courtesy of Xiaofeng Xin

    See Abreview

References for Anti-PPAR gamma antibody - ChIP Grade (ab45036)

This product has been referenced in:

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Human Tissue sections (Adipose)
Specification Adipose
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 1 µg
Specification Liver
Gel Running Conditions Reduced Denaturing
Blocking step Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Dr. Xiaofeng Xin

Verified customer

Submitted May 13 2013

DISCOUNT CODE: 100%ABR-00000
Expiration date: 06. January 2013

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