Anti-PPAR gamma antibody - ChIP Grade (ab45036)

Overview

  • Product name
    Anti-PPAR gamma antibody - ChIP Grade
    See all PPAR gamma primary antibodies
  • Description
    Rabbit polyclonal to PPAR gamma - ChIP Grade
  • Specificity
    Ab45036 detects peroxisome proliferator activated receptor (PPAR) gamma 2. This antibody does not detect PPAR alpha or PPAR delta. This sequence is from P37231-1 (Isoform 2), the sequence is not present in P37231-2 (Isoform 1) or P37231-3 (Isoform 3).
  • Tested applications
    Suitable for: ChIP, IP, IHC-P, ICC/IF, WB, Functional Studies, EMSA, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Non human primates
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human PPAR gamma 2 aa 1-16.
    Sequence:

    MGETLGDSPIDPESDSC


    (Peptide available as ab41762)

  • Positive control
    • NIH-3T3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab45036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 21247904
IP Use at an assay dependent concentration. PubMed: 21247904
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.
WB 1/500. Predicted molecular weight: 56 kDa.Can be blocked with Human PPAR gamma peptide (ab41762).

By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate.

Functional Studies Use at an assay dependent concentration. Ab45036 inhibits PPAR gamma 2 DNA binding.
EMSA Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • Tissue specificity
    Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • Involvement in disease
    Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIMT1 antibody
    • GLM1 antibody
    • NR1C3 antibody
    • Nuclear receptor subfamily 1 group C member 3 antibody
    • OTTHUMP00000185032 antibody
    • OTTHUMP00000185036 antibody
    • Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody
    • Peroxisome proliferator activated receptor gamma 1 antibody
    • Peroxisome Proliferator Activated Receptor gamma antibody
    • Peroxisome proliferator-activated receptor gamma antibody
    • PPAR gamma antibody
    • PPAR-gamma antibody
    • PPARG antibody
    • PPARG_HUMAN antibody
    • PPARG1 antibody
    • PPARG2 antibody
    • PPARgamma antibody
    see all

Images

  • Lane 1 :
    Lane 2 : Anti-PPAR gamma antibody - ChIP Grade (ab45036)

    Lane 1 : Protein Marker
    Lane 2 : Mouse liver tissue lysate at 1 µg

    Secondary
    Lane 2 : IRDye® 680RD Donkey anti-Rabbit


    Performed under reducing conditions.

    Predicted band size : 56 kDa


    Exposure time : 2 seconds

    This image is courtesy of Xiaofeng Xin

    See Abreview

  • ab45036 staining PPAR gamma in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1:200) for 1 hour at room temperature. A Dylight 680-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/1000).

  • All lanes : Anti-PPAR gamma antibody - ChIP Grade (ab45036) at 1/500 dilution

    Lane 1 : 3T3
    Lane 2 : 3T3-L1 differentiated day 7

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 56 kDa

    PPAR gamma is detected in 3T3-L1 Day 7 differentiated cell lysates with some background bands. No detection of PPAR gamma occurs in 3T3 cell lysate.

     

  • ab45036 positive staining PPAR gamma in Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence) (right) negative control in abscence of ab45036 (left). Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) over night at 4°C. A DyLight 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin (red) stained with red phalloidin and nuclei (blue) stainded with DAPI.

  • Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. The image at the top shows 3T3-L1 cells differntiated (for 7 days) where PPAR gamma is shown in green. The image below shows 3T3-L1 undifferentiated cells where no PPAR gamma is detected.

     

  • Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. 3T3-L1 cells providing positive signal have been differentiated for 7 days.

    PPAR gamma is shown in red, lipid droplets (that indicates the proper differentiation of the cells) are shown in green.

References

This product has been referenced in:
  • Hu J  et al. Tumor grade related expression of neuroglobin is negatively regulated by PPAR? and confers antioxidant activity in glioma progression. Redox Biol 12:682-689 (2017). WB, IHC . Read more (PubMed: 28410531) »
  • Lin CH  et al. (-)-Epicatechin-3-O-ß-D-allopyranoside from Davallia formosana prevents diabetes and dyslipidemia in streptozotocin-induced diabetic mice. PLoS One 12:e0173984 (2017). Read more (PubMed: 28333970) »

See all 17 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (Adipose)
Specification
Adipose
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
1 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Dr. Xiaofeng Xin

Verified customer

Submitted May 13 2013

DISCOUNT CODE: 100%ABR-00000
Expiration date: 06. January 2013

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