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Synthetic peptide corresponding to Human PPAR gamma 2 aa 1-16.
Our Abpromise guarantee covers the use of ab45036 in the following tested applications.
|ChIP||Use at an assay dependent concentration. PubMed: 21247904|
|IP||Use at an assay dependent concentration. PubMed: 21247904|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500. Predicted molecular weight: 56 kDa.Can be blocked with Human PPAR gamma peptide (ab41762).
By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate.
|Functional Studies||Use at an assay dependent concentration. Ab45036 inhibits PPAR gamma 2 DNA binding.|
|EMSA||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
PPAR gamma is detected in 3T3-L1 Day 7 differentiated cell lysates with some background bands. No detection of PPAR gamma occurs in 3T3 cell lysate.
Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. 3T3-L1 cells providing positive signal have been differentiated for 7 days.
PPAR gamma is shown in red, lipid droplets (that indicates the proper differentiation of the cells) are shown in green.
Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. The image at the top shows 3T3-L1 cells differntiated (for 7 days) where PPAR gamma is shown in green. The image below shows 3T3-L1 undifferentiated cells where no PPAR gamma is detected.
This image is courtesy of Xiaofeng Xin