Overview

  • Product nameAnti-P.pastoris antibody
  • Description
    Rabbit polyclonal to P.pastoris
  • SpecificityReacts with all antigens.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with Yeast (Pichia pastoris)
  • Immunogen

    Native, whole cells (P. pastoris)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purification notesab53737 purity is >95%
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-Fr
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    WB: 1/50 - 1/200.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevancePichia pastoris, a methylotrophic yeast, has been used as a heterologous protein expression system. It combines many of the benefits of E. coli expression with the advantages of expression in an eukaryotic system such as protein processing, folding, and posttranslational modifications. Pichia pastoris is capable of metabolising methanol as the sole carbon source. The first step in the metabolism of methanol is the oxidation of methanol to formaldehyde by the enzyme, alcohol oxidase. Expression of this enzyme, coded for by the AOX1 gene, is tightly regulated and induced by methanol to very high levels:- typically >30% of the total soluble protein in the cells.
    • Alternative names
      • Pichia pastoris antibody

    References for Anti-P.pastoris antibody (ab53737)

    ab53737 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"