Overview

  • Product nameAnti-PPFIBP1 antibody
    See all PPFIBP1 primary antibodies
  • Description
    Rabbit polyclonal to PPFIBP1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: SPTSPFMGSL RALHLVEDLR GLLEMMETDE KEGLRCQIPD STAETLVEWL, corresponding to amino acids 37-86 of Human PPFIBP1

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A.
  • Tissue specificityWidely expressed. Absent in liver.
  • Sequence similaritiesBelongs to the liprin family. Liprin-beta subfamily.
    Contains 3 SAM (sterile alpha motif) domains.
  • DomainThe N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type beta/beta. The C-terminal, non-coiled coil regions mediate heterodimerization type beta/alpha and interaction with S100A4.
  • Information by UniProt
  • Database links
  • Alternative names
    • PPFIBP 1 antibody
    • hSGT2 antibody
    • hSgt2p antibody
    • KIAA1230 antibody
    • L2 antibody
    • LIPB1_HUMAN antibody
    • Liprin beta 1 antibody
    • liprin related protein antibody
    • Liprin-beta-1 antibody
    • PPFIBP1 antibody
    • Protein tyrosine phosphatase receptor type f polypeptide interacting protein binding protein 1 antibody
    • Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1 antibody
    • PTPRF interacting protein binding protein 1 antibody
    • PTPRF interacting protein, binding protein 1 (liprin beta 1) antibody
    • PTPRF-interacting protein-binding protein 1 antibody
    • SGT2 antibody
    see all

Anti-PPFIBP1 antibody images

  • Anti-PPFIBP1 antibody (ab58605) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 19 kDa
    Observed band size : 19 kDa

References for Anti-PPFIBP1 antibody (ab58605)

ab58605 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293 cells)
Loading amount 50 µg
Specification 293 cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Melanie Blasius

Verified customer

Submitted Nov 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"