The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionMay regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A.
Tissue specificityWidely expressed. Absent in liver.
Sequence similaritiesBelongs to the liprin family. Liprin-beta subfamily. Contains 3 SAM (sterile alpha motif) domains.
DomainThe N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type beta/beta. The C-terminal, non-coiled coil regions mediate heterodimerization type beta/alpha and interaction with S100A4.