The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 53 kDa.
Use a concentration of 20 µg/ml.
Use at an assay dependent concentration.
FunctionInvolved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
Post-translational modificationsSubstrate of transglutaminase (in vitro).
Cellular localizationNucleus. Cytoplasm. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions.