Recombinant Anti-PPIL1 antibody [EPR10251] (ab154188)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10251] to PPIL1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PPIL1 antibody [EPR10251]
See all PPIL1 primary antibodies -
Description
Rabbit monoclonal [EPR10251] to PPIL1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562 cells and cell lysate, HeLa cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10251 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. |
Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. -
Tissue specificity
Ubiquitous, with the most abundant expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.
Contains 1 PPIase cyclophilin-type domain. - Information by UniProt
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Database links
- Entrez Gene: 51645 Human
- Omim: 601301 Human
- SwissProt: Q5TDC9 Human
- SwissProt: Q9Y3C6 Human
- Unigene: 27693 Human
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Alternative names
- CGI 124 antibody
- CGI-124 antibody
- CGI124 antibody
see all
Images
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All lanes : Anti-PPIL1 antibody [EPR10251] (ab154188) at 1/1000 dilution
Lane 1 : K562 cell lysates
Lane 2 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa -
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling PPIL1 using ab154188 at 1/10 dilution (red) or rabbit IgG negative control antibody (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154188 has not yet been referenced specifically in any publications.