Overview

  • Product name
  • Description
    Rabbit polyclonal to PPM1A
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-264 of Human PPM1A (Uniprot ID: P35813).

  • Positive control
    • 293T, A431 and Mouse brain whole cell lysates; Human colon carcinoma tissue; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling.
  • Sequence similarities
    Belongs to the PP2C family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein phosphatase 2C isoform alpha antibody
    • EC 3.1.3.16 antibody
    • FLJ42306 antibody
    • IA antibody
    • MGC9201 antibody
    • Mpp alpha antibody
    • PP2C alpha antibody
    • PP2C-alpha antibody
    • PP2CA antibody
    • PP2Calpha antibody
    • PPM 1A antibody
    • PPM1A antibody
    • PPM1A_HUMAN antibody
    • PPPM1A antibody
    • Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform antibody
    • Protein phosphatase 1A antibody
    • Protein phosphatase 1A magnesium dependent alpha antibody
    • Protein phosphatase 2C alpha antibody
    • Protein phosphatase 2C alpha isoform antibody
    • Protein phosphatase 2C isoform alpha antibody
    • Protein phosphatase IA antibody
    • Protein phosphatase, Mg2+/Mn2+ dependent, 1A antibody
    see all

Images

  • All lanes : Anti-PPM1A antibody (ab154489) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 42 kDa
  • Anti-PPM1A antibody (ab154489) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 42 kDa
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling PPM1A with ab154489 at 1/500 dilution.
  • Immunofluorescent analysis of methanol-fixed (iced cold; 5mins) A431 cells labeling PPM1A with ab154489 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.

References

ab154489 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154489.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up