Overview

  • Product nameAnti-PPM1B antibody
    See all PPM1B primary antibodies
  • Description
    Rabbit polyclonal to PPM1B
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 450 and the C-terminus (residue 479) of Human PPM1B (NP_002697.)

  • Positive control
    • Whole cell lysate from HeLa, 293T and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionEnzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro.
  • Tissue specificityHighly expressed in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the PP2C family.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC21657 antibody
    • OTTHUMP00000158953 antibody
    • OTTHUMP00000158954 antibody
    • OTTHUMP00000158955 antibody
    • OTTHUMP00000202394 antibody
    • OTTHUMP00000202395 antibody
    • OTTHUMP00000202399 antibody
    • PP2C beta antibody
    • PP2C beta X antibody
    • PP2C-beta antibody
    • PP2CB antibody
    • PP2Cbeta antibody
    • PPC2BETAX antibody
    • PPM 1B antibody
    • Ppm1b antibody
    • PPM1B_HUMAN antibody
    • Protein phosphatase 1B (formerly 2C) magnesium dependent beta isoform antibody
    • Protein phosphatase 1B antibody
    • Protein phosphatase 1B magnesium dependent beta isoform antibody
    • Protein phosphatase 2C beta isoform antibody
    • Protein phosphatase 2C isoform beta antibody
    • Protein phosphatase 2C like protein antibody
    • Protein phosphatase, Mg2+/Mn2+ dependent, 1B antibody
    see all

Anti-PPM1B antibody images

  • All lanes : Anti-PPM1B antibody (ab70804) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 53 kDa
    Observed band size : 53 kDa


    Exposure time : 3 minutes
  • Detection of PPM1B by Western Blot of Immunoprecipitate.
    1mg whole cell lysate from HeLa cells was immunoprecipitated using ab70804 at 3ug/mg of lysate. for the subsequent western blot, 20% of the immunoprecipitate was probed with ab70804 at 1ug/ml.
    Detection: chemiluminescence with exposure time of 10 seconds.

References for Anti-PPM1B antibody (ab70804)

ab70804 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

Read More

Thank you for your prompt response and for confirming the order details.

I have looked at our record and it seems that all the vials you purchased are from the same bleed and purification; so there is no difference between the previously purch...

Read More

Thank you for your enquiry regarding ab70804 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

As our Abpromise indicates, in the event that a pr...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"