Overview

  • Product name
  • Description
    Rabbit polyclonal to PPM1D
  • Specificity
    Targets C terminal of PPM1D.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PPM1D aa 571-602 (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: O15297

  • Positive control
    • 293 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was eluted out with both high and low pH buffers and neutralised immediately after elution then followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

Target

  • Function
    Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins.
  • Sequence similarities
    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.1.3.16 antibody
    • p53 induced protein phosphatase 1 antibody
    • p53-induced protein phosphatase 1 antibody
    • PP2C delta antibody
    • PP2C-delta antibody
    • PP2CD antibody
    • Ppm1d antibody
    • PPM1D_HUMAN antibody
    • Protein phosphatase 1D antibody
    • protein phosphatase 1D magnesium-dependent delta isoform antibody
    • Protein phosphatase 2C delta isoform antibody
    • Protein phosphatase 2C isoform delta antibody
    • Protein phosphatase magnesium dependent 1 delta antibody
    • Protein phosphatase magnesium-dependent 1 delta antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 1D antibody
    • Protein phosphatase Wip1 antibody
    • Wild type p53 induced phosphatase 1 antibody
    • WIP 1 antibody
    • WIP1 antibody
    see all

Images

  • Anti-PPM1D antibody (ab37495) + 293 cell lysate

    Predicted band size : 67 kDa
    Observed band size : 67 kDa

References

This product has been referenced in:
  • Ali AY  et al. The oncogenic phosphatase PPM1D confers cisplatin resistance in ovarian carcinoma cells by attenuating checkpoint kinase 1 and p53 activation. Oncogene : (2011). Read more (PubMed: 21927021) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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