Overview

  • Product nameAnti-PPM1D antibody
    See all PPM1D primary antibodies
  • Description
    Mouse monoclonal to PPM1D
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human PPM1D aa 496-605.
    Sequence:

    IGLVPTNSTNTVMDQKNLKMSTPGQMKAQEIERTPPTNFKRTLEESNSGP LMKKHRRNGLSRSSGAQPASLPTTSQRKNSVKLTMRRRLRGQKKIGNPLL HQHRKTVCVC


    Database link: O15297

Properties

Applications

Our Abpromise guarantee covers the use of ab56332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 67 kDa.

Target

  • FunctionRequired for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins.
  • Sequence similaritiesBelongs to the PP2C family.
    Contains 1 PP2C-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.1.3.16 antibody
    • p53 induced protein phosphatase 1 antibody
    • p53-induced protein phosphatase 1 antibody
    • PP2C delta antibody
    • PP2C-delta antibody
    • PP2CD antibody
    • Ppm1d antibody
    • PPM1D_HUMAN antibody
    • Protein phosphatase 1D antibody
    • protein phosphatase 1D magnesium-dependent delta isoform antibody
    • Protein phosphatase 2C delta isoform antibody
    • Protein phosphatase 2C isoform delta antibody
    • Protein phosphatase magnesium dependent 1 delta antibody
    • Protein phosphatase magnesium-dependent 1 delta antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 1D antibody
    • Protein phosphatase Wip1 antibody
    • Wild type p53 induced phosphatase 1 antibody
    • WIP 1 antibody
    • WIP1 antibody
    see all

Anti-PPM1D antibody images

  • Anti-PPM1D antibody (ab56332) at 5 µg/ml + 0.2 µg/lane of partial recombinant protein with a tag

    Secondary
    Goat anti mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 67 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PPM1D antibody (ab56332) at 5 µg/ml

    Lane 1 : 50 µg/lane of transfected 293T cell line lysate
    Lane 2 : 50 µg/lane of non-transfected 293T cell line lysate

    Secondary
    Goat anti mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 67 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

References for Anti-PPM1D antibody (ab56332)

ab56332 has not yet been referenced specifically in any publications.

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