Overview

  • Product name
  • Description
    Rabbit polyclonal to PPM1E
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    EVEGESLDLC LQQLYKYNCP SFLAAALARA TSDEVLQSDL SAHYIPKETD GTEGTVEIET VKLARSVFSK LHEI internal sequence amino acids 148-221 of Human PPM1E.

  • Positive control
    • Human cerebellum tissue (Purkinje cells and cells in the molecular layer), Human cell line U-2 OS.

Properties

Applications

Our Abpromise guarantee covers the use of ab121571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42.
  • Sequence similarities
    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.
  • Cellular localization
    Nucleus. Cytoplasm. A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ca(2+)/calmodulin-dependent protein kinase phosphatase N antibody
    • CaMKN antibody
    • CaMKP-N antibody
    • CaMKP-nucleus antibody
    • KIAA1072 antibody
    • Nuclear calmodulin dependent protein kinase phosphatase antibody
    • Partner of PIX 1 antibody
    • Partner of PIX-alpha antibody
    • Partner of PIXA antibody
    • POPX1 antibody
    • PP2CH antibody
    • Ppm1e antibody
    • PPM1E_HUMAN antibody
    • Protein phosphatase 1E antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 1E antibody
    see all

Images

  • ab121571, at a 1/10 dilution, staining PPM1E in Purkinje cells and in cells of the molecular layer of paraffin-embedded Human cerebellum tissue by immunohistochemistry.
  • ab121571, at 4 µg/ml, staining PPM1E in the nucleus & nucleoli of PFA/Triton X-100 treated Human cell line U-2 OS by immunofluorescence. Antibody staining is shown in green.

References

ab121571 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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