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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Function Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro).
Sequence similarities Belongs to the AB hydrolase superfamily.
Information by UniProt
PME 1 antibody
Anti-PPME1 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPME1 antibody (ab86409)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling PPME1 with ab86409 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - PPME1. Right - Merge.
Western blot - PPME1 antibody (ab86409)
Anti-PPME1 antibody (ab86409) at 1 µg/ml + 721_B cell lysate at 10 µg Secondary HRP-conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 42 kDa Gel concentration 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PPME1 antibody(ab86409)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cortex tissue labelling PPME1 with ab86409 at 5µg/ml.
References for Anti-PPME1 antibody (ab86409)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"