Overview

  • Product name
    Anti-PPO antibody
  • Description
    Rabbit polyclonal to PPO
  • Specificity
    ab65549 specifically reacts with a 64 kDa polyphenol oxidase from chloroplast thylakoid lumen.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with spinach.
  • Immunogen

    Recombinant lumenal polyphenol oxidase (AA 83 to 625) from spinach.

  • Positive control
    • Spinach chloroplast proteins.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).

Target

  • Relevance
    PPO, polyphenol oxidase, participates in the response of plants to wounding and herbivore attack, mediated by the octadecanoid wound signalling pathway. Chloroplast PPO is a 64 kDa nuclear encoded protein that is targeted to the thylakoid lumen. PPO is one of the most strongly phosphorylated proteins in thylakoid lumen although the role of this protein modification is not known.
  • Cellular localization
    Chloroplast thylakoid lumen.
  • Alternative names
    • Catechol oxidase antibody
    • polyphenol oxidase antibody

Anti-PPO antibody images

  • Anti-PPO antibody (ab65549) at 1/1000 dilution + Spinach chloroplast proteins at 5 µg

    Secondary
    Anti-rabbit at 30000
    Developed using the ECL technique

    Predicted band size : 64 kDa
    Observed band size : 64 kDa

References for Anti-PPO antibody (ab65549)

ab65549 has not yet been referenced specifically in any publications.

Product Wall

Thank you and your customer very much for your interest in ab65549.

To our knowledge, ab65549 has not been tested in Liliopsida .

I cannot find sequence data, but I believe that if PPO is expressed it should be very conserved sinc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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