Overview

  • Product nameAnti-PPOX antibody
    See all PPOX primary antibodies
  • Description
    Mouse monoclonal to PPOX
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: EASGCVLSQE LFQQRAQEAA ATQLGLKEMP SHCLVHLHKN CIPQYTLGHW QKLESARQFL TAHRLPLTLA GASYEGVAVN DCIESGRQAA VSVLGTEPNS , corresponding to amino acids 378-478 of Human PPOX

Properties

Applications

Our Abpromise guarantee covers the use of ab58147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
    • Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
    • Involvement in diseaseDefects in PPOX are the cause of variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. PV is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease.
    • Sequence similaritiesBelongs to the protoporphyrinogen oxidase family.
    • Cellular localizationMitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC8485 antibody
      • PPO antibody
      • PPOX antibody
      • PPOX_HUMAN antibody
      • Protoporphyrinogen oxidase antibody
      • V290M antibody
      • Variegate porphyria antibody
      • VP antibody
      see all

    Anti-PPOX antibody images

    • PPOX antibody (ab58147) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lung, adenosqumous cell carcinoma.
    • Western blot against tagged recombinant protein immunogen using ab58147 PPOX antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-PPOX antibody (ab58147)

    ab58147 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"