Recombinant
RabMAb

Anti-PPOX antibody [EPR10400] (ab170412)

Overview

  • Product name
    Anti-PPOX antibody [EPR10400]
    See all PPOX primary antibodies
  • Description
    Rabbit monoclonal [EPR10400] to PPOX
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPOX aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P50336

  • Positive control
    • HepG2, HT-1080, 293T and A549 cell lysates. A549 cells.Permeabilized HT-1080 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
    • Tissue specificity
      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Pathway
      Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
    • Involvement in disease
      Defects in PPOX are the cause of variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. PV is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease.
    • Sequence similarities
      Belongs to the protoporphyrinogen oxidase family.
    • Cellular localization
      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC8485 antibody
      • PPO antibody
      • PPOX antibody
      • PPOX_HUMAN antibody
      • Protoporphyrinogen oxidase antibody
      • V290M antibody
      • Variegate porphyria antibody
      • VP antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling PPOX with ab170412 at a 1/100 dilution (green). DAPI nuclear staining (blue).

    • Flow cytometric analysis of permeabilized HT-1080 cells using ab170412 (red) at a 1/100 dilution or a rabbit IgG (negative) (green).

    • All lanes : Anti-PPOX antibody [EPR10400] (ab170412) at 1/1000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : HepG2 cell lysates
      Lane 3 : HT-1080 cell lysates
      Lane 4 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 51 kDa

    References

    ab170412 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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