Recombinant
RabMAb

Anti-PPOX antibody [EPR10401] (ab170884)

Overview

  • Product name
    Anti-PPOX antibody [EPR10401]
    See all PPOX primary antibodies
  • Description
    Rabbit monoclonal [EPR10401] to PPOX
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPOX aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P50336

  • Positive control
    • HepG2, 293T, A549 and HT-1080 cell lysates, Human liver tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
    • Tissue specificity
      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Pathway
      Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
    • Involvement in disease
      Defects in PPOX are the cause of variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. PV is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease.
    • Sequence similarities
      Belongs to the protoporphyrinogen oxidase family.
    • Cellular localization
      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC8485 antibody
      • PPO antibody
      • PPOX antibody
      • PPOX_HUMAN antibody
      • Protoporphyrinogen oxidase antibody
      • V290M antibody
      • Variegate porphyria antibody
      • VP antibody
      see all

    Images

    • ab170884 showing +ve staining in Human normal brain tissue.

    • ab170884 showing +ve staining in Human cervical carcinoma tissue.

    • ab170884 showing +ve staining in Human normal kidney tissue.

    • ab170884 showing +ve staining in Human thyroid gland carcinoma tissue.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PPOX using ab170884 at a 1/50 dilution.

    • Lane 1 : Anti-PPOX antibody [EPR10401] (ab170884) at 1/1000 dilution
      Lanes 2-4 : Anti-cardiac Troponin I antibody (ab1000) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : HT1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 51 kDa

    References

    ab170884 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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