Overview

  • Product nameAnti-PPP1CA + 1CB antibody [EP1511Y]
  • Description
    Rabbit monoclonal [EP1511Y] to PPP1CA + 1CB
  • SpecificityThe immunogen for ab52619 is 100% homologous with Human PPP1CA and PPP1CB
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPP1CA + 1CB aa 200-300. The exact sequence is proprietary.
    Database link: P62140

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Human cerebrum cortex tissue. ICC/IF: HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Applications

Our Abpromise guarantee covers the use of ab52619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000 - 1/100000. Predicted molecular weight: 37 kDa.
IP 1/30 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol.

Flow Cyt 1/100 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/250.

Target

Anti-PPP1CA + 1CB antibody [EP1511Y] images

  • ab52619 staining PPP1CA + 1CB in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • All lanes : Anti-PPP1CA + 1CB antibody [EP1511Y] (ab52619) at 1/100000 dilution

    Lane 1 : Mouse Brain lysate
    Lane 2 : Rat Brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size : 37 kDa
  • ab52619 staining PP1CA + 1CB in Human cerebrum cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/50). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Overlay histogram showing HeLa cells stained with ab52619 (red line) at 1/150 dilution. The cells were fixed with 80% methanol. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)

  • ab52619 (purified) at 1/30 immunoprecipitating PPP1CA + 1CB in Jurkat cell lysate. For western blotting, a HRP-conjugated Goat anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-PPP1CA + 1CB antibody [EP1511Y] (ab52619) at 1/100000 dilution

    Lane 1 : T47-D cell Lysate
    Lane 2 : HeLa cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size : 37 kDa
  • Overlay histogram showing HeLa cells stained with ab52619, unpurified (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52619, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • All lanes : Anti-PPP1CA + 1CB antibody [EP1511Y] (ab52619) at 1/20000 dilution

    Lane 1 : SKBR-3 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size : 37 kDa

References for Anti-PPP1CA + 1CB antibody [EP1511Y] (ab52619)

This product has been referenced in:
  • Pribiag H & Stellwagen D TNF-a downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors. J Neurosci 33:15879-93 (2013). WB . Read more (PubMed: 24089494) »
  • Horiuchi D  et al. MYC pathway activation in triple-negative breast cancer is synthetic lethal with CDK inhibition. J Exp Med 209:679-96 (2012). Read more (PubMed: 22430491) »

See all 3 Publications for this product

Product Wall

Thank you for calling Abcam earlier today.

I blasted the epiopte sequence for ab52619 and I do not think it would recognize other isoforms. But below is the immunogen sequence, if you would like to double check:
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