Overview

  • Product nameAnti-PPP1R10 antibody
    See all PPP1R10 primary antibodies
  • Description
    Rabbit polyclonal to PPP1R10
  • SpecificityWe have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Other Immunogen Type corresponding to PPP1R10 aa 350-400.

  • Positive control
    • Nuclear extract from Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab70247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48, 115 kDa (predicted molecular weight: 99 kDa).
IP Use a concentration of 1 - 10 µg/ml.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevancePPP1R10 (serine/threonine protein phosphatase 1 regulatory subunit 10), also known as PNUTS, is a targeting subunit of protein phosphatase 1 (PP1). Furthermore, PPP1R10 acts as an inhibitor of PPP1CA and PPP1CC phosphatase activities. Its inhibitory activity on PPP1CA is only observed when PPP1R10 is phosphorylated by Protein Kinase A (PKA). PPP1R10 contains a zinc finger motif and is predominantly localised within the nucleus. Recently, PPP1R10 has been shown to target PP1 to GABA(C) receptors, shuttling from the nucleus to the cell membrane in cells co-expressing this receptor. This highlights a role for this protein in the regulation of synaptic signal transduction. PPP1R10 has also been shown to regulate the activity of p53 in response to hypoxia.
  • Cellular localizationNucleus. Note: Found in discrete nucleoplasmic bodies and within nucleoli.
  • Database links
  • Alternative names
    • CAT 53 antibody
    • CAT53 antibody
    • FB 19 antibody
    • FB19 antibody
    • FB19 protein antibody
    • HLA-C associated transcript 53 antibody
    • MHC class I region proline rich protein CAT53 antibody
    • p99 antibody
    • Phosphatase 1 nuclear targeting subunit antibody
    • Phosphatase nuclear targeting subunit antibody
    • PNUTS antibody
    • PP1 binding protein of 114 kDa antibody
    • PP1 nuclear targeting subunit antibody
    • PP1R10 antibody
    • protein FB19 antibody
    • Protein phosphatase 1 nuclear targeting subunit antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 10 antibody
    • Protein phosphatase 1 regulatory subunit 10 antibody
    • R111 antibody
    • Serine/threonine protein phosphatase 1 regulatory subunit 10 antibody
    see all

Anti-PPP1R10 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PPP1R10 with ab70247 at 1/1000 (1µg/ml). Detection: DAB.
  • Lanes 1 - 2 : Anti-PPP1R10 antibody (ab70247) at 1 µg/ml
    Lanes 3 - 4 : Anti-PPP1R10 antibody (ab70247) at 0.2 µg/ml

    Lane 1 : Nuclear extract from Hela cells at 15 µg
    Lane 2 : Nuclear extract from Hela cells at 45 µg
    Lane 3 : Nuclear extract from Hela cells at 15 µg
    Lane 4 : Nuclear extract from Hela cells at 45 µg


    Predicted band size : 99 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of PPP1R10
    Lane 1: Another antibody which recognizes a different epitope of PPP1R10.
    Lane 2: Another antibody which recognizes a different epitope of PPP1R10.
    Lane 3: ab70247 at 1µg/100µg nuclear extract.
    Lane 4: Another antibody which recognizes a different epitope of PPP1R10.
    Detection: Chemiluminescence with exposure times of 3 seconds.

References for Anti-PPP1R10 antibody (ab70247)

ab70247 has not yet been referenced specifically in any publications.

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