Overview

  • Product nameAnti-PPP1R10 antibody
    See all PPP1R10 primary antibodies
  • Description
    Rabbit polyclonal to PPP1R10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from within residues 450 - 550 of Human PPP1R10.

    (Peptide available as ab74588.)

  • Positive control
    • This antibody gave a positive signal in the following Nuclear Lysates: HeLa, HepG2, Jurkat.

Properties

Applications

Our Abpromise guarantee covers the use of ab74589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).

Target

  • RelevancePPP1R10 (serine/threonine protein phosphatase 1 regulatory subunit 10), also known as PNUTS, is a targeting subunit of protein phosphatase 1 (PP1). Furthermore, PPP1R10 acts as an inhibitor of PPP1CA and PPP1CC phosphatase activities. Its inhibitory activity on PPP1CA is only observed when PPP1R10 is phosphorylated by Protein Kinase A (PKA). PPP1R10 contains a zinc finger motif and is predominantly localised within the nucleus. Recently, PPP1R10 has been shown to target PP1 to GABA(C) receptors, shuttling from the nucleus to the cell membrane in cells co-expressing this receptor. This highlights a role for this protein in the regulation of synaptic signal transduction. PPP1R10 has also been shown to regulate the activity of p53 in response to hypoxia.
  • Cellular localizationNucleus. Note: Found in discrete nucleoplasmic bodies and within nucleoli.
  • Database links
  • Alternative names
    • CAT 53 antibody
    • CAT53 antibody
    • FB 19 antibody
    • FB19 antibody
    • FB19 protein antibody
    • HLA-C associated transcript 53 antibody
    • MHC class I region proline rich protein CAT53 antibody
    • p99 antibody
    • Phosphatase 1 nuclear targeting subunit antibody
    • Phosphatase nuclear targeting subunit antibody
    • PNUTS antibody
    • PP1 binding protein of 114 kDa antibody
    • PP1 nuclear targeting subunit antibody
    • PP1R10 antibody
    • protein FB19 antibody
    • Protein phosphatase 1 nuclear targeting subunit antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 10 antibody
    • Protein phosphatase 1 regulatory subunit 10 antibody
    • R111 antibody
    • Serine/threonine protein phosphatase 1 regulatory subunit 10 antibody
    see all

Anti-PPP1R10 antibody images

  • All lanes : Anti-PPP1R10 antibody (ab74589) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : HepG2 (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat nuclear extract lysate (ab14844)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

References for Anti-PPP1R10 antibody (ab74589)

ab74589 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"