Overview

  • Product nameAnti-PPP1R16A antibody
    See all PPP1R16A primary antibodies
  • Description
    Mouse polyclonal to PPP1R16A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-528 of Human PPP1R16A

  • Positive control
    • Human pancreas tissue lysate and 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 29, 58 and 75 kDa (predicted molecular weight: 58 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates.
    • Sequence similaritiesContains 5 ANK repeats.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2900084E10Rik antibody
      • Likley ortholog of mouse myosin phosphatase targeting subunit 3 antibody
      • MGC14333 antibody
      • Myosin phosphatase target subunit 3 antibody
      • Myosin phosphatase targeting subunit 3 antibody
      • Myosin phosphatase-targeting subunit 3 antibody
      • MYPT3 antibody
      • PP16A_HUMAN antibody
      • PPP1R16A antibody
      • Protein phosphatase 1 regulatory subunit 16A antibody
      • Protein phosphatase 1, regulatory (inhibitor) subunit 16A antibody
      • R75527 antibody
      see all

    Anti-PPP1R16A antibody images

    • Anti-PPP1R16A antibody (ab67194) at 1/500 dilution + human pancreas tissue lysate at 25 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 58 kDa
      Observed band size : 58 kDa
      Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-PPP1R16A antibody (ab67194) at 1/500 dilution

      Lane 1 : PPP1R16A transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 58 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    References for Anti-PPP1R16A antibody (ab67194)

    ab67194 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab67194.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"