Recombinant
RabMAb

Anti-PPP1R16B antibody [EPR10293(B)] (ab154834)

Overview

  • Product name
    Anti-PPP1R16B antibody [EPR10293(B)]
  • Description
    Rabbit monoclonal [EPR10293(B)] to PPP1R16B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human PPP1R16B (Uniprot: Q96T49).

  • Positive control
    • 293T and K562 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1. Involved in the regulation of endothelial cell filopodia extension. May be a downstream target for TGF-beta1 signaling cascade in endothelial cells.
    • Tissue specificity
      Highly expressed in vascular endothelium, CNS, lung, spleen, kidney and testis.
    • Sequence similarities
      Contains 5 ANK repeats.
    • Post-translational
      modifications
      Phosphorylated by PKA and, after PKA priming, by GSK3B. Phosphorylation by GSK3B reduces its association with PP1C and enhances PP1C activity. Dephosphorylation by its associated PP1C results in enhanced association with PP1C, but reduced PP1C activity.
    • Cellular localization
      Cell membrane. Cell membrane. Nucleus. Co-localizes with RPSA/LAMR1 in the cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANKRD4 antibody
      • Ankyrin repeat domain containing protein 4 antibody
      • ankyrin repeat domain protein 4 antibody
      • Ankyrin repeat domain-containing protein 4 antibody
      • CAAX box protein TIMAP antibody
      • hTIMAP antibody
      • KIAA0823 antibody
      • PP16B_HUMAN antibody
      • PPP1R16B antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 16B antibody
      • protein phosphatase 1, regulatory (inhibitor) subunit 16B antibody
      • TGF beta inhibited membrane associated protein antibody
      • TGF-beta-inhibited membrane-associated protein antibody
      • TIMAP antibody
      see all

    Images

    • All lanes : Anti-PPP1R16B antibody [EPR10293(B)] (ab154834) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 64 kDa

    References

    ab154834 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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