Overview

  • Product name
    Anti-PPP1R3D antibody
  • Description
    Rabbit polyclonal to PPP1R3D
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: ELAQVKVFNA GDDPSVPLHV LSRLAINSDL CCSSQDLEFT LHCLVPDFPP PVEAADFGER LQRQLVCLER VTCSDL, corresponding to amino acids 112-187 of Human PPP1R3D (O95685).

  • Positive control
    • Human rectum tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis.
  • Tissue specificity
    Expressed in all tissue tested. High expression in skeletal muscle and heart.
  • Sequence similarities
    Contains 1 CBM21 (carbohydrate binding type-21) domain.
  • Domain
    The CBM21 domain is known to be involved in the localization to glycogen and is characteristic of some regulatory subunit of phosphatase complexes.
  • Information by UniProt
  • Database links
  • Alternative names
    • PP1 subunit R6 antibody
    • PPP1R3D antibody
    • PPP1R6 antibody
    • PPR3D_HUMAN antibody
    • Protein phosphatase 1 regulatory subunit 3D antibody
    • Protein phosphatase 1 regulatory subunit 6 antibody
    • Protein phosphatase 1-binding subunit R6 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli, cytoplasm and mitochondria. Recommended concentration of ab122696 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122696, at 1/200 dilution, staining PPP1R3D in Paraffin-embedded Human rectum tissue by Immunohistochemistry

References

ab122696 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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