• Product nameAnti-PPP2CB antibody
    See all PPP2CB primary antibodies
  • Description
    Rabbit polyclonal to PPP2CB
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide from a region between residue 260 and the C-terminus (residue 309) of human PPP2CB, NP_002706.1.

  • Positive control
    • Whole cell lysate from Hela, NIH3T3 and 293T cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab72343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects bands of approximately 28, 36 and 190 kDa (predicted molecular weight: 36 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
    • Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
    • Post-translational
      Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
      Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
    • Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
    • Information by UniProt
    • Database links
    • Alternative names
      • PP2A beta antibody
      • PP2A-beta antibody
      • PP2AB_HUMAN antibody
      • PP2Abeta antibody
      • PP2CB antibody
      • Ppp2cb antibody
      • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody
      • Protein phosphatase 2 catalytic subunit beta isozyme antibody
      • Protein phosphatase 2, catalytic subunit, beta isoform antibody
      • Protein phosphatase 2A catalytic subunit beta isoform antibody
      • Protein phosphatase type 2A catalytic subunit antibody
      • Serine/threonine protein phosphatase 2A catalytic subunit beta antibody
      • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
      see all

    Anti-PPP2CB antibody images

    • All lanes : Anti-PPP2CB antibody (ab72343) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : 36 kDa
      Additional bands at : 190 kDa,28 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Immunoprecipitation/ Western Blot of PPP2CB.
      Lane 1: ab72343 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72343 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-PPP2CB antibody (ab72343)

    ab72343 has not yet been referenced specifically in any publications.

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