Purification notesAffinity purified using an epitope specific to PP2A immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Application notesIs unsuitable for IP.
FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
Post-translational modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro). Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
All lanes : Anti-PPP2CB antibody (ab72450) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 36 kDa Observed band size : 36 kDa Additional bands at : 100 kDa,28 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
References for Anti-PPP2CB antibody (ab72450)
has not yet been referenced specifically in any publications.
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