Overview

  • Product nameAnti-PPP2CB antibody
    See all PPP2CB primary antibodies
  • Description
    Rabbit polyclonal to PPP2CB
  • SpecificityThis antibody recognizes a region of PPP2CB that is highly conserved with PPP2CA
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Dog, Ferret, Zebrafish, Rhesus monkey, Gorilla
  • Immunogen

    A synthetic peptide corresponding to a region between residues 1 and 50 of human PPP2CB.

  • Positive control
    • HeLa whole cell lysate; 293T and mouse NIH3T3 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified using an epitope specific to PP2A immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionPP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
    • Sequence similaritiesBelongs to the PPP phosphatase family. PP-1 subfamily.
    • Post-translational
      modifications
      Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
      Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
    • Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
    • Information by UniProt
    • Database links
    • Alternative names
      • PP2A beta antibody
      • PP2A-beta antibody
      • PP2AB_HUMAN antibody
      • PP2Abeta antibody
      • PP2CB antibody
      • Ppp2cb antibody
      • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody
      • Protein phosphatase 2 catalytic subunit beta isozyme antibody
      • Protein phosphatase 2, catalytic subunit, beta isoform antibody
      • Protein phosphatase 2A catalytic subunit beta isoform antibody
      • Protein phosphatase type 2A catalytic subunit antibody
      • Serine/threonine protein phosphatase 2A catalytic subunit beta antibody
      • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
      see all

    Anti-PPP2CB antibody images

    • All lanes : Anti-PPP2CB antibody (ab72450) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 36 kDa
      Observed band size : 36 kDa
      Additional bands at : 100 kDa,28 kDa,75 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes

    References for Anti-PPP2CB antibody (ab72450)

    ab72450 has not yet been referenced specifically in any publications.

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