Anti-PPP2R1A antibody (ab154551)

Overview

  • Product name
  • Description
    Rabbit polyclonal to PPP2R1A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 308-589 of PPP2R1A (Uniprot ID P30153)

  • Positive control
    • HeLa, NIH-3T3, JC, BCL-1 whole cell lysate; HeLa cells; U87 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

  • Function
    The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.
  • Sequence similarities
    Belongs to the phosphatase 2A regulatory subunit A family.
    Contains 15 HEAT repeats.
  • Domain
    Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
  • Cellular localization
    Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2AAA_HUMAN antibody
    • 6330556D22Rik antibody
    • Alpha isoform of regulatory subunit A, protein phosphatase antibody
    • DKFZp470L0914 antibody
    • Medium tumor antigen-associated 61 kDa protein antibody
    • MGC128399 antibody
    • MGC786 antibody
    • MGC83000 antibody
    • MGC95083 antibody
    • PP2A Aalpha antibody
    • PP2A antibody
    • PP2A subunit A isoform PR65-alpha antibody
    • PP2A subunit A isoform R1-alpha antibody
    • PP2A, subunit A, PR65-alpha isoform antibody
    • PP2A, subunit A, R1-alpha isoform antibody
    • PP2AAALPHA antibody
    • Ppp2r1a antibody
    • ppp2r1a-b antibody
    • PR65 antibody
    • PR65-alpha antibody
    • PR65A antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform antibody
    • Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A, antibody
    • Protein phosphatase 2 regulatory subunit A alpha antibody
    • Protein phosphatase 2, 65-KD regulatory subunit A, alpha antibody
    • Protein phosphatase 2, regulatory subunit A, alpha isoform antibody
    • Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A antibody
    • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody
    see all

Images

  • Anti-PPP2R1A antibody (ab154551) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 65 kDa



    7.5% SDS PAGE
  • All lanes : Anti-PPP2R1A antibody (ab154551) at 1/5000 dilution

    Lane 1 : NIH-3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 65 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling PPP2R1A with ab154551 antibody at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded U87 xenograft labeling PPP2R1A with ab154551 antibody at 1/500 dilution.

References

ab154551 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 19 2015


Here is the IHC testing protocol that was used.

The laboratory used Tris-EDTA buffer as antigen retrieval, but the suitable buffer may be dependent on different tissue. We recommend the customer try Tris-EDTA buffer and citrate buffer, an...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up