Anti-PPP2R1A antibody (ab154551)
Key features and details
- Rabbit polyclonal to PPP2R1A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-PPP2R1A antibody
See all PPP2R1A primary antibodies -
Description
Rabbit polyclonal to PPP2R1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Zebrafish, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to a region within amino acids 308-589 of PPP2R1A (Uniprot ID P30153)
-
Positive control
- HeLa, NIH-3T3, JC, BCL-1 whole cell lysate; HeLa cells; U87 xenograft
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
|
|
ICC/IF | (1) |
1/100 - 1/1000.
|
IHC-P |
1/100 - 1/1000.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 65 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
Target
-
Function
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. -
Sequence similarities
Belongs to the phosphatase 2A regulatory subunit A family.
Contains 15 HEAT repeats. -
Domain
Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure. -
Cellular localization
Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1. - Information by UniProt
-
Database links
- Entrez Gene: 535321 Cow
- Entrez Gene: 5518 Human
- Entrez Gene: 51792 Mouse
- Entrez Gene: 397088 Pig
- Entrez Gene: 117281 Rat
- Entrez Gene: 406685 Zebrafish
- Omim: 605983 Human
- SwissProt: Q32PI5 Cow
see all -
Alternative names
- 2AAA_HUMAN antibody
- 6330556D22Rik antibody
- Alpha isoform of regulatory subunit A, protein phosphatase antibody
see all
Images
-
Anti-PPP2R1A antibody (ab154551) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 65 kDa
7.5% SDS PAGE -
All lanes : Anti-PPP2R1A antibody (ab154551) at 1/5000 dilution
Lane 1 : NIH-3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PPP2R1A with ab154551 antibody at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
-
Immunohistochemical analysis of paraffin-embedded U87 xenograft labeling PPP2R1A with ab154551 antibody at 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab154551 has been referenced in 2 publications.
- Elgenaidi IS & Spiers JP Hypoxia modulates protein phosphatase 2A through HIF-1a dependent and independent mechanisms in human aortic smooth muscle cells and ventricular cardiomyocytes. Br J Pharmacol 176:1745-1763 (2019). PubMed: 30825189
- Lombardi LM et al. An RNA interference screen identifies druggable regulators of MeCP2 stability. Sci Transl Med 9:N/A (2017). PubMed: 28835516