The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 65 kDa.
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionThe PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.
Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit A family. Contains 15 HEAT repeats.
DomainEach HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
Cellular localizationCytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1.
ab31316 (3.8µg/ml) shows staining of PPP2R1A in paraffin embedded Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows textured cytoplasm and membranous staining in the epithelial cells.
Western blot - PPP2R1A antibody (ab31316)
Anti-PPP2R1A antibody (ab31316) at 0.03 µg/ml + Human Lymph Node lysate (35µg protein in RIPA buffer)