Overview

  • Product name
  • Description
    Goat polyclonal to PPP2R1A
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-KYFAQEALTVLSLA

    , corresponding to C terminal amino acids 575-588 of Human PPP2R1A.

  • Positive control
    • Human Thymus and Lymph Node lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000.
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 65 kDa.
IHC-P Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.
  • Sequence similarities
    Belongs to the phosphatase 2A regulatory subunit A family.
    Contains 15 HEAT repeats.
  • Domain
    Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
  • Cellular localization
    Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2AAA_HUMAN antibody
    • 6330556D22Rik antibody
    • Alpha isoform of regulatory subunit A, protein phosphatase antibody
    • DKFZp470L0914 antibody
    • Medium tumor antigen-associated 61 kDa protein antibody
    • MGC128399 antibody
    • MGC786 antibody
    • MGC83000 antibody
    • MGC95083 antibody
    • PP2A Aalpha antibody
    • PP2A antibody
    • PP2A subunit A isoform PR65-alpha antibody
    • PP2A subunit A isoform R1-alpha antibody
    • PP2A, subunit A, PR65-alpha isoform antibody
    • PP2A, subunit A, R1-alpha isoform antibody
    • PP2AAALPHA antibody
    • Ppp2r1a antibody
    • ppp2r1a-b antibody
    • PR65 antibody
    • PR65-alpha antibody
    • PR65A antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform antibody
    • Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A, antibody
    • Protein phosphatase 2 regulatory subunit A alpha antibody
    • Protein phosphatase 2, 65-KD regulatory subunit A, alpha antibody
    • Protein phosphatase 2, regulatory subunit A, alpha isoform antibody
    • Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A antibody
    • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody
    see all

Images

  • ab31316 (3.8µg/ml) shows staining of PPP2R1A in paraffin embedded Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows textured cytoplasm and membranous staining in the epithelial cells.

  • Anti-PPP2R1A antibody (ab31316) at 0.03 µg/ml + Human Lymph Node lysate (35µg protein in RIPA buffer)

    Predicted band size : 65 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

References

ab31316 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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