The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
FunctionThe PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.
Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit A family. Contains 15 HEAT repeats.
DomainEach HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
Cellular localizationCytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1.