Anti-PPP2R1A antibody - Aminoterminal end (ab76697)

Overview

  • Product name
    Anti-PPP2R1A antibody - Aminoterminal end
    See all PPP2R1A primary antibodies
  • Description
    Rabbit polyclonal to PPP2R1A - Aminoterminal end
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide selected from the N-terminal region of human PPP2R1A conjugated to KLH

  • Positive control
    • Jurkat, T47D, and HepG2 cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab76697 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • Function
    The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.
  • Sequence similarities
    Belongs to the phosphatase 2A regulatory subunit A family.
    Contains 15 HEAT repeats.
  • Domain
    Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
  • Cellular localization
    Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2AAA_HUMAN antibody
    • 6330556D22Rik antibody
    • Alpha isoform of regulatory subunit A, protein phosphatase antibody
    • DKFZp470L0914 antibody
    • Medium tumor antigen-associated 61 kDa protein antibody
    • MGC128399 antibody
    • MGC786 antibody
    • MGC83000 antibody
    • MGC95083 antibody
    • PP2A Aalpha antibody
    • PP2A antibody
    • PP2A subunit A isoform PR65-alpha antibody
    • PP2A subunit A isoform R1-alpha antibody
    • PP2A, subunit A, PR65-alpha isoform antibody
    • PP2A, subunit A, R1-alpha isoform antibody
    • PP2AAALPHA antibody
    • Ppp2r1a antibody
    • ppp2r1a-b antibody
    • PR65 antibody
    • PR65-alpha antibody
    • PR65A antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform antibody
    • Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A, antibody
    • Protein phosphatase 2 regulatory subunit A alpha antibody
    • Protein phosphatase 2, 65-KD regulatory subunit A, alpha antibody
    • Protein phosphatase 2, regulatory subunit A, alpha isoform antibody
    • Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A antibody
    • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody
    see all

Images

  • All lanes : Anti-PPP2R1A antibody - Aminoterminal end (ab76697) at 1/60 dilution

    Lane 1 : Jurkat cell line lysate
    Lane 2 : T47D cell line lysate
    Lane 3 : HepG2 cell line lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

References

ab76697 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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