Validated using a knockout cell line
Recombinant
RabMAb

Anti-PPP2R1B antibody [EPR10159] (ab154833)

Overview

  • Product name
    Anti-PPP2R1B antibody [EPR10159]
    See all PPP2R1B primary antibodies
  • Description
    Rabbit monoclonal [EPR10159] to PPP2R1B
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human PPP2R1B (UniProt: P30154).

  • Positive control
    • Caco2 and Jurkat cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10159
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
    • Sequence similarities
      Belongs to the phosphatase 2A regulatory subunit A family.
      Contains 15 HEAT repeats.
    • Domain
      Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2AAB_HUMAN antibody
      • beta isoform of regulatory subunit A, protein phosphatase 2 antibody
      • PP2A A beta antibody
      • PP2A subunit A isoform PR65-beta antibody
      • PP2A subunit A isoform R1-beta antibody
      • PP2A, subunit A, PR65 beta isoform antibody
      • PP2A, subunit A, R1 beta isoform antibody
      • PPP2R1B antibody
      • PPP4R1 antibody
      • PR65B antibody
      • protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform antibody
      • protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform antibody
      • Protein phosphatase 2, regulatory subunit A, beta antibody
      • Protein phosphatase 2, structural/regulatory subunit A, beta antibody
      • protein phosphatase 2, structural/regulatory subunit A, beta isoform antibody
      • serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform antibody
      • serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform antibody
      • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform antibody
      see all

    Images



    • Predicted band size : 66 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: Caco2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab154833 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab154833 was shown to recognize PPP2R1B when PPP2R1B knockout samples were used, along with additional cross-reactive bands. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab154833 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-PPP2R1B antibody [EPR10159] (ab154833) at 1/1000 dilution

      Lane 1 : Caco2 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 66 kDa

    References

    ab154833 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    We have not tested 7288-1 or 7301-1 for cross reactivity with PP2AA alpha, however, we have only observed one band in our in-house testing with 7288-1 and we have detected only 2 isoforms of PP2AA beta with 7301-1. It is possible they could react, base...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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