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Synthetic peptide, corresponding to residues in Human PPP2R1B (UniProt: P30154).
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab154833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 66 kDa.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PPP2R1B knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: Caco2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab154833 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab154833 was shown to recognize PPP2R1B when PPP2R1B knockout samples were used, along with additional cross-reactive bands. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab154833 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab154833 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"