The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: Use at a concentration of 2 - 10 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 19762425).
IP: Use at an assay dependent dilution (PMID 19762425).
WB: Use at a concentration of 5 - 10 µg/ml. Detects a band of approximately 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Tissue specificityExpressed in all tissues examined.
Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit B family. Contains 7 WD repeats.
Anti-PPP2R2A antibody (ab28370) (5-10 ug/ml) + Total rat brain homogenate (20-50ug)
References for Anti-PPP2R2A antibody (ab28370)
This product has been referenced in:
O'Shaughnessy RF et al. Akt-dependent Pp2a activity is required for epidermal barrier formation during late embryonic development. Development136:3423-31 (2009).
WB, IF, IHC-Fr, IP
Read more (PubMed: 19762425) »