Recombinant
RabMAb

Anti-PPP2R2D antibody [EPR13624] (ab181071)

Overview

  • Product name
    Anti-PPP2R2D antibody [EPR13624]
    See all PPP2R2D primary antibodies
  • Description
    Rabbit monoclonal [EPR13624] to PPP2R2D
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human PPP2R2D aa 50-200. The exact sequence is proprietary.
    Database link: Q66LE6

  • Positive control
    • HeLa, SH-SY5Y and Human fetal brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment.
    • Sequence similarities
      Belongs to the phosphatase 2A regulatory subunit B family.
      Contains 7 WD repeats.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2ABD_HUMAN antibody
      • MDS026 antibody
      • PP2A subunit B isoform B55-delta antibody
      • PP2A subunit B isoform delta antibody
      • PP2A subunit B isoform PR55-delta antibody
      • PP2A subunit B isoform R2-delta antibody
      • PPP2R2D antibody
      • Protein phosphatase 2 regulatory subunit B delta antibody
      • Protein phosphatase 2 regulatory subunit B delta isoform antibody
      • Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform antibody
      see all

    Images

    • Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg

      Secondary
      HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 52 kDa
      Observed band size: 52 kDa



      Blocking Buffer: 5% NFDM/TBST

      Dilution Buffer: 5% NFDM/TBST

    • Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution (purified) + Mouse brain tissue lysate

      Secondary
      HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 52 kDa
      Observed band size: 52 kDa



      Blocking Buffer: 5% NFDM/TBST

      Dilution Buffer: 5% NFDM/TBST

    • All lanes : Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/1000 dilution (purified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : Human fetal brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 52 kDa
      Observed band size: 52 kDa



      Blocking Buffer: 5% NFDM/TBST

      Dilution Buffer: 5% NFDM/TBST

    • All lanes : Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/2000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 52 kDa
      Observed band size: 52 kDa

    References

    ab181071 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab181071.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up