Overview

  • Product name
  • Description
    Rabbit polyclonal to PPP2R3A
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 921-1129 of Human PPP2R3A (Q06190).

  • Positive control
    • NT2D1, U-87 MG and MCF7 whole cell lysates; Human Colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab126195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 130 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • Tissue specificity
    Expressed in heart, brain, placenta, lung, muscle and kidney.
  • Sequence similarities
    Contains 2 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI118493 antibody
    • DNA for thyroid hormone receptor binding site (258bp) antibody
    • P2R3A_HUMAN antibody
    • PP2A subunit B B''-PR72/PR130 antibody
    • PP2A subunit B B72/B130 isoforms antibody
    • PP2A subunit B isoform PR72/PR130 antibody
    • PP2A subunit B isoform R3 isoform antibody
    • PP2A subunit B isoforms B''-PR72/PR130 antibody
    • PP2A subunit B isoforms B72/B130 antibody
    • PP2A subunit B PR72/PR130 isoforms antibody
    • PP2A subunit B R3 isoform antibody
    • PPP2R3 antibody
    • Ppp2r3a antibody
    • PR130 antibody
    • PR59 antibody
    • PR72 antibody
    • Protein phosphatase 2 (formerly 2A) regulatory subunit B'' alpha antibody
    • Serine/threonine protein phosphatase 2A 72/130 kDa regulatory subunit B antibody
    • Serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B antibody
    • Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha antibody
    see all

Images

  • All lanes : Anti-PPP2R3A antibody (ab126195) at 1/1000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : U-87 MG whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 130 kDa
  • ab126195, at 1/250 dilution, staining PPP2R3A in Paraffin-embedded Human Colon carcinoma tissue by Immunohistochemistry.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MEF, NB4, HCT-116)
Gel Running Conditions
Reduced Denaturing (8% / 12% step gel)
Loading amount
40 µg
Specification
MEF, NB4, HCT-116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Anke Rauch

Verified customer

Submitted Mar 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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