Overview

  • Product name
  • Description
    Goat polyclonal to PPP2R5A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide: AYNMHSILSNTSAE, corresponding to C terminal amino acids 473-486 of Human PPP2R5A.

  • Positive control
    • Human Heart and Muscle lysate.
  • General notes
    GenBank Accession Number – NP_006234.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human PPP2R5A peptide (ab22797).

Target

  • Function
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • Tissue specificity
    Widely expressed with the highest expression in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the phosphatase 2A regulatory subunit B56 family.
  • Post-translational
    modifications
    Phosphorylated on serine residues.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome > centromere. From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2A5A_HUMAN antibody
    • B56A antibody
    • MGC131915 antibody
    • PP2A B subunit B' alpha isoform antibody
    • PP2A B subunit B56 alpha isoform antibody
    • PP2A B subunit isoform B''-alpha antibody
    • PP2A B subunit isoform B56-alpha antibody
    • PP2A B subunit isoform PR61-alpha antibody
    • PP2A B subunit isoform R5-alpha antibody
    • PP2A B subunit PR61 alpha isoform antibody
    • PP2A B subunit R5 alpha isoform antibody
    • PPP2R5A antibody
    • PR61A antibody
    • PR61alpha antibody
    • Protein phosphatase 2 regulatory subunit B (B56) alpha isoform antibody
    • Protein phosphatase 2 regulatory subunit B' alpha antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform antibody
    see all

Images



  • Predicted band size : 55 kDa

References

This product has been referenced in:
  • Mannava S  et al. PP2A-B56a controls oncogene-induced senescence in normal and tumor human melanocytic cells. Oncogene 31:1484-92 (2012). Read more (PubMed: 21822300) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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