Overview

  • Product name
  • Description
    Rabbit polyclonal to PPP2R5A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 108-157 (VSTNRGVIVE SAYSDIVKMI SANIFRTLPP SDNPDFDPEE DEPTLEASWP) of human PPP2R5A (NP_006234).

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • Tissue specificity
    Widely expressed with the highest expression in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the phosphatase 2A regulatory subunit B56 family.
  • Post-translational
    modifications
    Phosphorylated on serine residues.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome > centromere. From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2A5A_HUMAN antibody
    • B56A antibody
    • MGC131915 antibody
    • PP2A B subunit B' alpha isoform antibody
    • PP2A B subunit B56 alpha isoform antibody
    • PP2A B subunit isoform B''-alpha antibody
    • PP2A B subunit isoform B56-alpha antibody
    • PP2A B subunit isoform PR61-alpha antibody
    • PP2A B subunit isoform R5-alpha antibody
    • PP2A B subunit PR61 alpha isoform antibody
    • PP2A B subunit R5 alpha isoform antibody
    • PPP2R5A antibody
    • PR61A antibody
    • PR61alpha antibody
    • Protein phosphatase 2 regulatory subunit B (B56) alpha isoform antibody
    • Protein phosphatase 2 regulatory subunit B' alpha antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform antibody
    see all

Images

  • Anti-PPP2R5A antibody (ab86551) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

References

ab86551 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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