Overview

  • Product nameAnti-PPP2R5A antibody
    See all PPP2R5A primary antibodies
  • Description
    Mouse polyclonal to PPP2R5A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human PPP2R5A (NP_006234.1)

  • Positive control
    • Human placenta tissue lysate Jurkat cell lysate Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.

Target

  • FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • Tissue specificityWidely expressed with the highest expression in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit B56 family.
  • Post-translational
    modifications
    Phosphorylated on serine residues.
  • Cellular localizationCytoplasm. Nucleus. Chromosome > centromere. From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2A5A_HUMAN antibody
    • B56A antibody
    • MGC131915 antibody
    • PP2A B subunit B' alpha isoform antibody
    • PP2A B subunit B56 alpha isoform antibody
    • PP2A B subunit isoform B''-alpha antibody
    • PP2A B subunit isoform B56-alpha antibody
    • PP2A B subunit isoform PR61-alpha antibody
    • PP2A B subunit isoform R5-alpha antibody
    • PP2A B subunit PR61 alpha isoform antibody
    • PP2A B subunit R5 alpha isoform antibody
    • PPP2R5A antibody
    • PR61A antibody
    • PR61alpha antibody
    • Protein phosphatase 2 regulatory subunit B (B56) alpha isoform antibody
    • Protein phosphatase 2 regulatory subunit B' alpha antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform antibody
    see all

Anti-PPP2R5A antibody images

  • Anti-PPP2R5A antibody (ab89621) at 1 µg/ml + Human placenta tissue lysate at 50 µg

    Predicted band size : 56 kDa
  • Anti-PPP2R5A antibody (ab89621) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 56 kDa
  • All lanes : Anti-PPP2R5A antibody (ab89621) at 1 µg/ml

    Lane 1 : PPP2R5A transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)

References for Anti-PPP2R5A antibody (ab89621)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (CCRF-CEM cells)
Specification CCRF-CEM cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Nov 20 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step 5% BSA + 1% NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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Submitted Jul 09 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 35 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Human Cell lysate - nuclear (HeLa nuclear cell lysate)
Specification HeLa nuclear cell lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted May 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"