PPP2R5A overexpression 293T lysate (whole cell) (ab94164)

Overview

  • Product namePPP2R5A overexpression 293T lysate (whole cell)
  • Description
    PPP2R5A overexpression lysate
  • General notesab94164 is a 293T cell transfected lysate in which Human PPP2R5A has been transiently over-expressed using a pCMV-PPP2R5A plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Tissue specificity: Widely expressed with the highest expression in heart and skeletal muscle. Similarity: Belongs to the phosphatase 2A regulatory subunit B56 family. PTM: Phosphorylated on serine residues.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

PPP2R5A overexpression 293T lysate (whole cell) images

  • ab94164 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-PPP2R5A antibody (ab89621) at 1/500 dilution

    Lane 1 : PPP2R5A overexpression 293T lysate (whole cell) (ab94164)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for PPP2R5A overexpression 293T lysate (whole cell) (ab94164)

ab94164 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"