Overview

  • Product nameAnti-PPP2R5C antibody
    See all PPP2R5C primary antibodies
  • Description
    Rabbit polyclonal to PPP2R5C
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 144-193 (FLESPDFQPN IAKKYIDQKF VLQLLELFDS EDPRERDFLK TTLHRIYGKF) of Human PPP2R5C (NP_848703).

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab94633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.
  • Tissue specificityHighest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.
  • Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit B56 family.
  • Post-translational
    modifications
    Isoform Gamma-3 is phosphorylated on serine residues. Isoform Gamma-1 phosphorylation by ERK2 is IER3-dependent and inhibits ERK dephosphorylation by PP2A-PPP2R5C.
  • Cellular localizationNucleus. Chromosome > centromere.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2A5G_HUMAN antibody
    • B' alpha regulatory subunit antibody
    • B56G antibody
    • KIAA0044 antibody
    • MGC23064 antibody
    • PP2A B subunit B' gamma isoform antibody
    • PP2A B subunit B56 gamma isoform antibody
    • PP2A B subunit isoform B' gamma antibody
    • PP2A B subunit isoform B''-gamma antibody
    • PP2A B subunit isoform B56-gamma antibody
    • PP2A B subunit isoform PR61-gamma antibody
    • PP2A B subunit isoform R5-gamma antibody
    • PP2A B subunit PR61 gamma isoform antibody
    • PP2A B subunit R5 gamma isoform antibody
    • Ppp2r5c antibody
    • PR61G antibody
    • Protein phosphatase 2 regulatory subunit B (B56) gamma isoform antibody
    • Protein phosphatase 2 regulatory subunit B' gamma antibody
    • Protein phosphatase 2, regulatory subunit B', gamma isoform antibody
    • Renal carcinoma antigen NY REN 29 antibody
    • Renal carcinoma antigen NY-REN-29 antibody
    • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit gamma isoform antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform antibody
    see all

Anti-PPP2R5C antibody images

  • Anti-PPP2R5C antibody (ab94633) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
  • All lanes : Anti-PPP2R5C antibody (ab94633) at 1/500 dilution

    Lane 1 : Human cardiac tissue lysate
    Lane 2 : Canine cardiac tissue lysate
    Lane 3 : Rat cardiac tissue lysate
    Lane 4 : Mouse cardiac tissue lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    HRP-conjugated donkey anti-rabbit IgG

    Predicted band size : 61 kDa

    Image from DeGrande ST et al., The Journal of Biological Chemistry, 288, 1032-1046. January 11, 2013. Fig 2.; doi: 10.1074/jbc.M112.426957. Epub 2012 Nov 30.

References for Anti-PPP2R5C antibody (ab94633)

This product has been referenced in:
  • Camilleri M  et al. Pilot study of small bowel mucosal gene expression in patients with irritable bowel syndrome with diarrhea. Am J Physiol Gastrointest Liver Physiol 311:G365-76 (2016). Read more (PubMed: 27445342) »
  • DeGrande ST  et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem 288:1032-46 (2013). WB ; Human, Mouse, Rat, Dog . Read more (PubMed: 23204520) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryo)
Specification 0-4hr embryo
Treatment control shRNA and DPPP2R5C (PP2A-B') shRNA embryos
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Richelle Sopko

Verified customer

Submitted Sep 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Organ, 293 cell)
Loading amount 25 µg
Specification Organ, 293 cell
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"