Overview

  • Product nameAnti-PPP2R5D antibody
    See all PPP2R5D primary antibodies
  • Description
    Rabbit polyclonal to PPP2R5D
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human PPP2R5D (NP_006236.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • Tissue specificityIsoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
  • Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit B56 family.
  • Cellular localizationCytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2A5D_HUMAN antibody
    • B'delta antibody
    • B56D antibody
    • Delta isoform of regulatory subunit B56, protein phosphatase 2A antibody
    • MGC2134 antibody
    • MGC8949 antibody
    • OTTHUMP00000039821 antibody
    • PP2A B subunit B' delta isoform antibody
    • PP2A B subunit B56 delta isoform antibody
    • PP2A B subunit isoform B''-delta antibody
    • PP2A B subunit isoform B56-delta antibody
    • PP2A B subunit isoform PR61-delta antibody
    • PP2A B subunit isoform R5-delta antibody
    • PP2A B subunit PR61 delta isoform antibody
    • PP2A B subunit R5 delta isoform antibody
    • PPP2R5D antibody
    • Protein phosphatase 2 regulatory subunit B (B56) delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B' delta antibody
    • Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • TEG-271 antibody
    • Tex271 antibody
    see all

Anti-PPP2R5D antibody images

  • All lanes : Anti-PPP2R5D antibody (ab72303) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 67 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • Detection of Human PPP2R5D by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72303 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 10 seconds. seconds

References for Anti-PPP2R5D antibody (ab72303)

ab72303 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"