Recombinant
RabMAb

Anti-PPP2R5D antibody [EPR15617-50] (ab188325)

Overview

  • Product name
    Anti-PPP2R5D antibody [EPR15617-50]
    See all PPP2R5D primary antibodies
  • Description
    Rabbit monoclonal [EPR15617-50] to PPP2R5D
  • Host species
    Rabbit
  • Specificity
    Based on sequence analysis, ab188325 recognizes 3 isoforms with the predicted MWs of 58kDa, 66kDa and 70kDa, respectively
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human PPP2R5D aa 450-600. The exact sequence is proprietary.
    Database link: Q14738

  • Positive control
    • C6, HepG2, HeLa, Jurkat and 293T cell lysates; Human brain and astrocytoma tissues; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 70 kDa.
IHC-P 1/50 - 1/100.

Perform heat mediated antigen retrieval using EDTA Buffer, pH9 before commencing with IHC staining protocol.

IP 1/40 - 1/60.

Target

  • Function
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • Tissue specificity
    Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
  • Sequence similarities
    Belongs to the phosphatase 2A regulatory subunit B56 family.
  • Cellular localization
    Cytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2A5D_HUMAN antibody
    • B'delta antibody
    • B56D antibody
    • Delta isoform of regulatory subunit B56, protein phosphatase 2A antibody
    • MGC2134 antibody
    • MGC8949 antibody
    • OTTHUMP00000039821 antibody
    • PP2A B subunit B' delta isoform antibody
    • PP2A B subunit B56 delta isoform antibody
    • PP2A B subunit isoform B''-delta antibody
    • PP2A B subunit isoform B56-delta antibody
    • PP2A B subunit isoform PR61-delta antibody
    • PP2A B subunit isoform R5-delta antibody
    • PP2A B subunit PR61 delta isoform antibody
    • PP2A B subunit R5 delta isoform antibody
    • PPP2R5D antibody
    • Protein phosphatase 2 regulatory subunit B (B56) delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B' delta antibody
    • Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • TEG-271 antibody
    • Tex271 antibody
    see all

Images

  • All lanes : Anti-PPP2R5D antibody [EPR15617-50] (ab188325) at 1/50000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PPP2R5D with ab188325 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling PPP2R5D with ab188325 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Western blot analysis of PPP2R5D in 293T cell lysate immunoprecipitated with ab188325 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1500 was used as secondary antibody.

References

ab188325 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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