Overview

  • Product name
    Anti-PPP2R5D antibody [EPR15617]
    See all PPP2R5D primary antibodies
  • Description
    Rabbit monoclonal [EPR15617] to PPP2R5D
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human PPP2R5D aa 450-600. The exact sequence is proprietary.
    Database link: Q14738

  • Positive control
    • C6, HepG2, Jurkat, HeLa, 293T cell lysate; Human brain tissue; HepG2 cells; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 58, 66, 70 kDa (predicted molecular weight: 70 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250.
IP 1/40 - 1/60.

Target

  • Function
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • Tissue specificity
    Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
  • Sequence similarities
    Belongs to the phosphatase 2A regulatory subunit B56 family.
  • Cellular localization
    Cytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2A5D_HUMAN antibody
    • B'delta antibody
    • B56D antibody
    • Delta isoform of regulatory subunit B56, protein phosphatase 2A antibody
    • MGC2134 antibody
    • MGC8949 antibody
    • OTTHUMP00000039821 antibody
    • PP2A B subunit B' delta isoform antibody
    • PP2A B subunit B56 delta isoform antibody
    • PP2A B subunit isoform B''-delta antibody
    • PP2A B subunit isoform B56-delta antibody
    • PP2A B subunit isoform PR61-delta antibody
    • PP2A B subunit isoform R5-delta antibody
    • PP2A B subunit PR61 delta isoform antibody
    • PP2A B subunit R5 delta isoform antibody
    • PPP2R5D antibody
    • Protein phosphatase 2 regulatory subunit B (B56) delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B' delta antibody
    • Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • TEG-271 antibody
    • Tex271 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PPP2R5D using ab188323 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

  • All lanes : Anti-PPP2R5D antibody [EPR15617] (ab188323) at 1/100000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    Based on the sequence analysis, ab188323 recognizes 3 isoforms with the predicted MWs of 58KDa, 66KDa and 70KDa, respectively.

  • Immunoprecipitation analysis of 293T cell lysate labeling PPP2R5D using ab188323 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 was used as secondary antibody.

  • Immunofluorescence analysis of HepG2 cells labeling PPP2R5D using ab188323 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde. Counterstain: DAPI.

  • Flow cytometry analysis of HeLa cells labeling PPP2R5D using ab188323 at 1/40 dilution (pink). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green).

References

This product has been referenced in:
  • Greenwood EJ  et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). Read more (PubMed: 27690223) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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