Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab1083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWestern Blot – Approx 55kDa and 40kDa bands seen in Mouse Brain extracts. Recommended for use at 0.5-2µg/ml.

    Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271(36):22081-9).
  • Target

    • FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
    • Sequence similaritiesBelongs to the phosphatase 2A regulatory subunit B56 family.
    • Post-translational
      modifications
      Phosphorylated on serine residues.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2A5E_HUMAN antibody
      • Epsilon isoform of regulatory subunit B56 protein phosphatase 2A antibody
      • PP2A B subunit B' epsilon antibody
      • PP2A B subunit B' epsilon isoform antibody
      • PP2A B subunit B56 epsilon antibody
      • PP2A B subunit B56 epsilon isoform antibody
      • PP2A B subunit isoform B''-epsilon antibody
      • PP2A B subunit isoform B'-epsilon antibody
      • PP2A B subunit isoform B56-epsilon antibody
      • PP2A B subunit isoform PR61-epsilon antibody
      • PP2A B subunit isoform R5-epsilon antibody
      • PP2A B subunit PR61 epsilon antibody
      • PP2A B subunit PR61 epsilon isoform antibody
      • PP2A B subunit R5 epsilon antibody
      • PP2A B subunit R5 epsilon isoform antibody
      • PPP2R5E antibody
      • Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform antibody
      • Protein phosphatase 2 regulatory subunit B' epsilon antibody
      • Protein phosphatase 2 regulatory subunit B' epsilon isoform antibody
      • Regulatory subunit B of protein phosphatase 2 epsilon isoform antibody
      • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon antibody
      • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform antibody
      see all

    Anti-PPP2R5E antibody images

    • Staining (0.5µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    References for Anti-PPP2R5E antibody (ab1083)

    ab1083 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample Mouse Cell (Oocyte)
    Specification Oocyte
    Permeabilization Yes - Triton X-100 0.1%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 29 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Organ, 293 cell)
    Loading amount 25 µg
    Specification Organ, 293 cell
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"