Overview

  • Product nameAnti-PPP4C antibody
    See all PPP4C primary antibodies
  • Description
    Goat polyclonal to PPP4C
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide:

    C-PQETRGIPSKKP

    , corresponding to C terminal amino acids 290-301 of Human PPP4C

  • Positive control
    • Human small intestine tissue; Human prostate tissue; HEK293 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionProtein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
  • Sequence similaritiesBelongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • PP X antibody
    • PP-X antibody
    • Pp4 antibody
    • PP4C antibody
    • PP4C_HUMAN antibody
    • PPH3 antibody
    • PPP4 antibody
    • ppp4c antibody
    • PPX antibody
    • protein phosphatase 4 (formerly X), catalytic subunit antibody
    • Protein phosphatase 4 catalytic subunit antibody
    • Protein phosphatase X antibody
    • protein phosphatase X, catalytic subunit antibody
    • Serine/threonine protein phosphatase 4 catalytic subunit antibody
    • Serine/threonine-protein phosphatase 4 catalytic subunit antibody
    see all

Anti-PPP4C antibody images

  • ab115741 at 2.5ug/ml staining PPP4C in Human small intestine tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115741 at 2.5ug/ml staining PPP4C in Human prostate tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-PPP4C antibody (ab115741) at 0.3 µg/ml + HEK293 cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 35 kDa

References for Anti-PPP4C antibody (ab115741)

ab115741 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"