• Product nameAnti-PPP4C antibody
    See all PPP4C primary antibodies
  • Description
    Goat polyclonal to PPP4C
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Xenopus laevis, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 290-301 of Human PPP4C (NP_002711.1).

  • Positive control
    • HEK293, A431 and HeLa cell lysates.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab87672 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab87672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.


  • FunctionProtein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
  • Sequence similaritiesBelongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • PP X antibody
    • PP-X antibody
    • Pp4 antibody
    • PP4C antibody
    • PP4C_HUMAN antibody
    • PPH3 antibody
    • PPP4 antibody
    • ppp4c antibody
    • PPX antibody
    • protein phosphatase 4 (formerly X), catalytic subunit antibody
    • Protein phosphatase 4 catalytic subunit antibody
    • Protein phosphatase X antibody
    • protein phosphatase X, catalytic subunit antibody
    • Serine/threonine protein phosphatase 4 catalytic subunit antibody
    • Serine/threonine-protein phosphatase 4 catalytic subunit antibody
    see all

Anti-PPP4C antibody images

  • Immunohistochemical staining of paraffin embedded human prostate with ab87672 at 2.5 µg/mL. Heat mediated antigen retrieval was performed with citrate buffer at pH 6.

  • All lanes : Anti-PPP4C antibody (ab87672) at 0.3 µg/ml

    Lane 1 : rat spleen tissue lysate
    Lane 2 : rat lung tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-PPP4C antibody (ab87672) at 0.3 µg/ml + HEK293 lysate (in RIPA buffer) at 35 µg
    developed using the ECL technique

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Primary incubation was 1 hour.

References for Anti-PPP4C antibody (ab87672)

ab87672 has not yet been referenced specifically in any publications.

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