Overview

  • Product name
  • Description
    Rabbit polyclonal to PPP6C
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N-terminal region of human PPP6C.

  • Positive control
    • Thymus cell lysate or human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab60249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Catalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7.
  • Tissue specificity
    Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ92648 antibody
    • MGC12249 antibody
    • PP 6 antibody
    • PP6 antibody
    • PP6C antibody
    • PPP 6 antibody
    • PPP 6C antibody
    • PPP6 antibody
    • PPP6_HUMAN antibody
    • Ppp6c antibody
    • Protein phosphatase 6 catalytic subunit antibody
    • Serine/threonine protein phosphatase 6 antibody
    • Serine/threonine protein phosphatase catalytic subunit antibody
    • Serine/threonine-protein phosphatase 6 catalytic subunit antibody
    see all

Images

  • Anti-PPP6C antibody (ab60249) at 1/100 dilution + Thymus cell lysate

    Predicted band size : 35 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Ab60249 (1/50-1/100) staining human PPP6C in human breast carcinoma tissue by immunohsitochemistry using formalin fixed, paraffin embedded tissue.

    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    BC = breast carcinoma

References

ab60249 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

Please let me know which antibody you would like to try inArabidopsis (ab70634 or ab60249), so that I can send you the testing discount code.

I look forward to hear back from you.

Use our pro...

Read More

Thank you for contacting Abcam. I have confirmed with the laboratory that neither ab60249 nor ab70634 share significant homology with the Drosophila sequence.  We do not expect these antibodies to cross react with this species. I apologize for ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up