• Product name
  • Description
    Rabbit polyclonal to PPP6C
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 250 and the C-terminus (residue 305) of Human PPP6C (NP_002712.1)

  • Positive control
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab70634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function
    Catalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7.
  • Tissue specificity
    Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ92648 antibody
    • MGC12249 antibody
    • PP 6 antibody
    • PP6 antibody
    • PP6C antibody
    • PPP 6 antibody
    • PPP 6C antibody
    • PPP6 antibody
    • PPP6_HUMAN antibody
    • Ppp6c antibody
    • Protein phosphatase 6 catalytic subunit antibody
    • Serine/threonine protein phosphatase 6 antibody
    • Serine/threonine protein phosphatase catalytic subunit antibody
    • Serine/threonine-protein phosphatase 6 catalytic subunit antibody
    see all


  • All lanes : Anti-PPP6C antibody (ab70634) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa

    Exposure time: 3 minutes
  • Detection of PPP6C by Western Blot of Immunoprecipitate. ab70634 at 1µg/ml staining PPP6C in HeLa whole cell lysate immunoprecipitated using ab70634 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.


This product has been referenced in:
  • Gallegos LL  et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). Read more (PubMed: 27255161) »

See 1 Publication for this product

Customer reviews and Q&As

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I have now raised an order to have the anti-PPP6C antibody (ab131335) to be sent to you free of charge. This is on the order number xxxxx (Purchase order number FOCR xxxxxx). Unfortunately this antibody is curre...

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Please let me know which antibody you would like to try inArabidopsis (ab70634 or ab60249), so that I can send you the testing discount code.

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Thank you for contacting Abcam. I have confirmed with the laboratory that neither ab60249 nor ab70634 share significant homology with the Drosophila sequence.  We do not expect these antibodies to cross react with this species. I apologize for ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Cell lysate - whole cell (fibroblast)
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

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Submitted Aug 07 2009


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