Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 178 kDa).

Target

  • RelevancePPRC1 acts as a coactivator during transcriptional activation of nuclear genes related to mitochondrial biogenesis and cell growth. It is involved in the transcription coactivation of CREB and NRF1 target genes.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Peroxisome proliferator-activated receptor gamma coactivator-related protein 1 antibody
    • PGC-1-related coactivator antibody
    • PRC antibody

Anti-PPRC1 antibody images

  • Anti-PPRC1 antibody (ab80900) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 178 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

References for Anti-PPRC1 antibody (ab80900)

ab80900 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted May 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"