Overview

  • Product nameAnti-PPT1 antibody
    See all PPT1 primary antibodies
  • Description
    Rabbit polyclonal to PPT1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH-conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human PPT1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • FunctionRemoves thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
  • Involvement in diseaseDefects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD).
  • Sequence similaritiesBelongs to the palmitoyl-protein thioesterase family.
  • Cellular localizationLysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
    • CLN1 antibody
    • EC 3.1.2.22 antibody
    • INCL antibody
    • Palmitoyl protein hydrolase 1 antibody
    • Palmitoyl protein thioesterase 1 antibody
    • Palmitoyl-protein hydrolase 1 antibody
    • Palmitoyl-protein thioesterase 1 antibody
    • PPT antibody
    • PPT-1 antibody
    • PPT1 antibody
    • PPT1_HUMAN antibody
    see all

Anti-PPT1 antibody images

  • Anti-PPT1 antibody (ab38417) at 1/100 dilution + 293 cell lysate.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References for Anti-PPT1 antibody (ab38417)

ab38417 has not yet been referenced specifically in any publications.

Product Wall

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As requested, I have issued a free of charge replacement of ab89022.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this fr...

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Thank you for your reply and for trying out my suggestions.


I think you are making some progress diluting out the secondary antibody. The background is way too high for a secondary antibody and to properly assess the results. I do notice ...

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Thank you for your reply.


I appreciate that you tested the suggestions made to improve your results. It is very alarming how much background you observe in the absence of primary. As a next step, it is necessary to further dilute the seco...

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Es freut mich sehr zu hören, dass ab38417 nun guteErgebnisse liefertund wünsche Ihnen viel Erfolg mit Ihrer Forschung.

Thank you for your reply.


I would recommend that you also test some higher dilutions of antibody (test 1:250 and 1:500) to help eliminate some background bands. Additionally, I would recommend using 5% BSA rather than milk to help reduce ...

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Thank you for your reply with this information.


The results of your WB with the human liver tissue are very promising, likely reflective of the band of interest as well as some glycosylated protein. What dilutions of antibody did you test...

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Thank you for contacting Abcam regarding ab38417.

I am sorry that you have been experiencing difficulties with this antibody in WB. In order to better understand the problems you are having in WB I would like to get some additional information...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"